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AIM To evaluate the possibility of dibenzoylmethane(DBM)on prevention and treatment of N,N-dimethylformamide(DMF)toxicosis.METHODS Animal experimental models of hepatotoxicity induced by DMF were applied to investigate the preventive and remedial effects respectively.In the preventive experiment,male ICR mice were administered(ig)with DBM 200,400 and 800 mg·kg-1·d-1,respectively,for 5 d.DMF(2.5 g·kg-1)was given(ip)into the mice 30 min after DBM administration on d 4.In the remedial experiment,male ICR mice were given(ip)DMF(2.5 g·kg-1),and 30 min later followed by DBM(ig)100,200 and 400 mg·kg-1·d-1 for 2 d.The mice serum was prepared and the mice were then euthanized after ip DMF for 48 h.The activities of serum glutamic-pyruvic transaminase(GPT),glutamic-oxaloacetic transaminase(GOT)and lactate dehydrogenase(LDH)were determined,and the liver tissues were collected for histopathological assessment(HE staining)under light microscope.The levels of glutathione(GSH)and glutathione disulfide in liver homogenate were also quantified.RESULTS After the administration of DMF,the activities of serum GPT,GOT and LDH were significantly increased,and the degeneration and necrosis in liver tissue were observed.In contrast,the activities of these serum enzymes were dominantly decreased in both DBM pretreatment group and posttreatment group compared to that in DMF model group.The histopathological changes in liver were also significantly ameliorated after pretreatment and posttreatment of DBM.Additionally,the content of GSH in liver homogenate was markedly reduced.CONCLUSION DBM had an obvious protective effect against DMF-induced acute hepatotoxicity in mice.


Post time: Apr-15-2021